Enzymology

But du cours

• To understand the mechanisms of enzymatic catalysis and the control of enzymatic kinetics.

• To be able to use enzymatic reactions to interpret cellular observations in relation to biochemical and physical compartmentalization.

• To be able to apply knowledge in enzymology to understand food degradation, certain biotechnology applications, and metabolic assays.

Acquis d'apprentissage visés

• Master the vocabulary of enzymology (enzyme, substrate, product, enzymatic reaction, cofactor, coenzyme, activator, inhibitor, allostery).

• Know the classification of enzymes.

• Understand the principles of enzymatic catalysis: equilibrium, free energy and activation energy, active site and reaction mechanism, specificity of the enzymatic reaction.

• Be able to monitor an enzymatic reaction.

• Enzyme kinetics: single-substrate reaction, Michaelis–Menten equation, kinetic parameters and linearization methods.

• Influence of enzyme or substrate concentration.

• Enzyme activity, specific activity, purification rate and yield of an enzyme.

• Influence of pH and temperature on enzyme activity.

• Understand the inhibition of enzymatic reactions: competitive, non-competitive, uncompetitive.

• Cooperativity and allostery.

Prérequis

Molecules of living organisms and bioenergetics

Programme

1. Introduction to general enzymology.

1. Classification of enzymes.

1. Kinetics of single-substrate enzymatic reactions.

1. Influence of environmental conditions.

1. Inhibition.

1. Cooperativity and allostery.

Modalités d'évaluation

2 Written exams (2h/2h)