But du cours
- Preparation of the project integrating microbiology, molecular biology, biochemistry and enzymology.
- Experimental implementation and data collection.
- Interpretation of results and formulation of perspectives.
Acquis d'apprentissage visés
- Conduct a situation analysis: gather, exploit, analyze and process information, then prioritize it.
- Structure reasoning by mobilizing relevant knowledge and mastering causal relationships; build an argumentation.
- Explore perspectives, provide a critical outlook and develop independent thinking.
Prérequis
- Basic knowledge in microbiology (culture, types of microorganisms).
- Fundamental concepts in molecular biology (DNA, genes, expression).
- Basics in protein biochemistry (structure, enzymatic activity).
- Ability to work under sterile conditions and comply with laboratory safety rules.
Programme
- The PadC protein from Bacillus subtilis:
- Bacterial cloning.
- Bacterial transformation.
- Recombinant expression.
- Extraction.
- Enzyme purification.
- Enzyme characterization.
Modalités d'évaluation
2 Written exams (2h/2h) + 2 oral presentations